Click to edit Master text styles,Second level,Third level,Fourth level,Fifth level,Click to edit Master title style,*,调谐页面,TQD,调谐页面,设定离子模式Ion mode is set,设定仪器参数Instrument parameters are set.,API 和 碰撞气Collision gases 已经调节完毕.,先导化合物Parent compounds已经确定并优化,子离子Daughter ions已经决定并优化,设置Mass 分辨率Resolution,碰撞能量Collision Energies已优化,执行采集,2,仪器调谐页面,(MS Tune),使用仪器快捷菜单Instrument,Shortcut Item,Accessing the TQD Tune Page,3,TQD,仪器调谐页面,4,保存调谐页面参数,Use the,New,Open,Save,or,Print,tools or use the File menu for these operations.,5,离子模式,Ion Mode,6,氮气与碰撞气,Nitrogen Gas and Collision Gas,7,Operate,Standby,Tune Page,源的控制界面,8,设置毛细管Capillary和锥孔Cone电压,9,设置萃取锥孔Extractor电压和RF透镜RF Lens电压,10,设置源Source以及去溶剂Desolvation温度,11,氮气流速,12,调谐页面,质量分析器,(Analyzer),13,常用的质量分析器参数设置,-MS Scan,14,常用的质量分析器参数设置,SIR,15,常用的质量分析器参数设置,-Daughter Scan,16,常用的质量分析器参数设置,MRM,17,常用的质量分析器参数设置,-Parent Scan,18,典型的质量分析器参数设置,Constant Neutral Loss,19,Purge,Refill Syringe,Start/Stop,流路Fluidics 控制,20,正常的流路,21,Infusion Dispense,22,Infusion Refill,23,Combined Infusion,24,Combined Refill,25,废液流路,Waste Flow,26,仪器调谐页面上的峰显示,27,显示质量数,(Da),的范围或者宽度,显示的中心,质量数,显示的信号增益,MS Scan,等功能的显示,峰显示设置,28,在底部或者顶部的工具栏双击鼠标，可以增加或者减少增益的设置,点击,X,一下，可以恢复前面的范围和增益设置，再点击一下可以回到缺省的设置。,峰显示设置,29,可以同时显示多个质谱峰,点击选择,是否显示,30,功能,Function,显示,31,Set=Parent/precursor Ion,Set,是母离子,/,先导离子,峰显示的设置,32,范围参数Scope Parameters,33,真空Vacuum状态,34,从调谐页面采集质谱图,35,从调谐页面采集质谱图,36,多离子模式,-ESCi,37,ESCi,调谐页面,38,Inter-Scan,设置,39,系统管理,40,诊断,41,查看仪器阈值参数Instrument Threshold Parameters,42,16 data points/amu,8 data points/amu,4 data points/amu,100 101 102 103 104,100%,94%,97%,Best,Resolution,Smooth Appearance,Compromise,m/z,M,M+1,Profile Data,数据点的格式,43,16 Data Points/Dalton,No Smoothing,8 Data Points/Dalton,No Smoothing,4 Data Points/Dalton,No Smoothing,Prostaglandin PGF2a-ESI negative,Acquisition Mode:Continuum,Mass range:100-500 amu,Scan Speed:400 amu/s,数据点,/Da,的数量对表观分辨率的影响,44,BASELINE LEVEL=0,0,Intensity,m/z,BASELINE LEVEL=1,0,Intensity,BASELINE LEVEL=5,0,Intensity,Ion Current X,Ion Current Z,Ion Current Y,Ion Current Y,Ion Current X,Baseline Level typically set to 0,Profile Data 基线水平Baseline Level,45,Centroid Data,46,单一离子记录Single Ion Recording，SIR:基线水平Baseline Level的影响,47,Set to,0,(Ion Counting Threshold Disabled),Set to,2,(2 time the RSD of noise observed without ion beam),Set to,4,(4 times the RSD of noise observed without ion beam),Set to,10,(10 times the RSD of noise observed without ion beam),Higher Intensity,Lower Intensity,Best Adjustment,Ion Counting Threshold,48,100,0,%,Spike Percentage,Ratio 50%,Data Point,Minimum Spike,Intensity,m/z,Intensity,Spike,去除毛刺Spike Removal,49,