单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,*,*,Antenna between electrodesusing EAG Probe with,Electrically conductive gel,Antennaholder,PROBE(Input amplifier),Antenna,Mounting the antenna using electrically conductive gel,Antennaholder,A,B,C,Example of Electroantennogram(EAG)Recording,Increasing concentration of stimulus,Quality of the EAG signal,Signal to Noise ratio,Sources of NOISE,Electronicnoise FILTER,Basic EAG stimulation arrangement,Stimulus source(Pasteur pipette)preparation,1,2,3,4,5,EAG signal quality,Stimulus duration,Effect of filter setting,EAG,D,ata evaluation,RECORDING by means of a RADAR BEAM,Portable SSR recording unit,Position of insect is recorded by an optical position sensor,BEHAVIOURALRESPONSE,Colorado potato beetle with 2x2 mm reflector,Portable EAG sensor on transport case,Pheromone concentration measurements,Example of Electroantennogram(EAG)Recording,SIGNAL DETECTION SIGNAL TRANSDUCTION,EAG recording of 21 stimulations,GC-EAD or GC-SSR,insect prep.,Antennaholder,Stimulus source(Pasteur pipette)preparation,EAG signal quality,Action potentials(spikes),EAG recording of 21 stimulations,Decline of antennal sensitivity over time,Normalization of EAG responses,Ref,Ref,Ref,Ref,Ref,Ref,EAG,I,n the field,M,ultiple antennae,C,ombined with Air sensor,RECORDING IN THE FIELD,using a portable EAG recording device,Antenna,compartment,Antenna,holder,Gel,Antenna,Gel,Portable EAG sensor on transport case,11,Pheromone concentration measurements,in 4 greenhouses,1,2,3,4,Pheromone concentration initially high,then declines,and increases again.,may,april,june,july,aug,sept,oct,nov,12,MULTIPLE ANTENNA EAG RECORDING,MULTIPLE(4)ANTENNA PROBE,4 antennae in,parallel,:,INCREASED,SENSITIVITY,(Signal-Noise Ratio),1,2,3,4,13,antenna,micro thermistor,MULTIMODAL SIGNAL RECORDING,EAG combined with air speed sensor,EAG signal,Air,speed,14,GC-EAD,E,le,ctro,A,ntenno,D,etection,G,as-,C,hromatography,COMBINED WITH GAS CHROMATOGRAPHY 1,15,PROBE(Input amplifier),Basic EAG stimulation arrangement,ACTIVITY PATTERN RECORDING(Whole insect or body parts),Application example,Pheromone concentration measurements,TEST ARENA,insect prep.,sphere 30 cm,Adult tick,Signal to Noise ratio,Referenceelectrode,Basic EAG stimulation arrangement,Single Sensillum Recording(SSR),Coupled Gas Chromatography-ElectroantennoDetection(GC-EAD),GC-EAD or GC-SSR,GC,transfer line,insect prep.,EAG,FID,COMBINED WITH GAS CHROMATOGRAPHY 2,16,COMBINED WITH GAS CHROMATOGRAPHY 3,Typical example,FID,EAG,17,SSR,S,ingle,S,ensillum,R,ecording,RECORDING through DIRECT CONTACT:,2.Single Sensillum Recording(SSR),AMPLIFIER,Action potentials(spikes),STIMULUS,Microelectrode makes contact with a single sensillum,Microelectrode,Referenceelectrode,Action potentials (spikes)are generated in response to an active compound,single sensillum,Antenna,10,PROBE(Input amplifier),COMBINED WITH GAS CHROMATOGRAPHY 3,INCREASED SENSITIVITY(Signal-Noise Ratio),Portable EAG sensor on transport case,BEHAVIOURALRESPONSE,INCREASED SENSITIVITY(Signal-Noise Ratio),Referenceelectrode,compartment,RECORDING by means of a RADAR BEAM,Antenna between electrodesusing EAG Probe with Electrically conductive gel,Mounting the antenna using electrically conductive gel,SIGNAL PROCESSOR,Effect of filter setting,Displacement of insect is analysed by a signal processor,COMBINED WITH GAS CHROMATOGRAPHY 1,RECORDING by means of a RADAR BEAM,PROBE(Input amplifier),S,ingle,S,ensillum,R,ecording IN THE FIELD,using a portable device,Antennal olfactory neuron activity,Air velocity,Laboratory SSR setup,Portable SSR recording unit,20 cm,18,Recording,Behavioural,Responses,SIGNAL DETECTION SIGNAL TRANSDUCTION,STIMULUS,SIGNALPROCESSING,SIGNALRESPONSE,(POWER SUPPLY),BEHAVIOURALRESPONSE,ELECTRICAL SIGNAL,ACTIVITY SIGNAL,RAW or PROCESSED SIGNAL?,19,RECORDING by means of a RADAR BEAM,ACTIVITY PATTERN RECORDING(Whole insect or body parts),20,RECORDING by means of a RADAR BEAM,Tobacco odour detection by conditioned(programmed)bee,Application example,21,RECORDING using VIDEO PROCESSING,1.Locomotion recording and analysis,VIDEO CAMERA,SIGNAL PROCESSOR,COMPUTER,Track Analyser,TEST ARENA,(4-Way OLFACTOMETER),22,RECORDING using OPTICAL DISPLACEMENT SENSOR,The servo-sphere or Locomotion Compensator,Two-dimensional,locomotion compensator,(Kramer,1976),Position of insect is recorded by an optical position sensor,Displacement of insect is analysed by a signal processor,Command signals are sent to the X-and Y-motors to,counter-act,the displacement of the insect.,23,RECORDING using VIDEO PROCESSING 1,Locomotion recording using the servo-sphere,video sensor,sphere 30 cm,Hungry Colorado potato beetle looking for a potato plant,24,RECORDING using VIDEO PROCESSING 2,Locomotion recording using a,mini,servo-sphere,1,2.,Tick nymph,1 mm,1.,Adult tick,(I.Ricinus),3 mm,Application of a,reflector,is difficult on small animals and strongly interferes with subsequent behaviour on the sphere,1 mm,Tick nymph,Colorado potato beetle with 2x2 mm reflector